On Setsuna's side, the humor tends to come from how obvious (if not explicit) her attraction to Konoka is, in spite of her staunch denial. That said, much of the humor surrounding Konoka and Setsuna's relationship comes less from the mere fact that they are implied to have feelings for each other, and more from their interactions.It has become a Running Gag for misinterpretations of situations the two get into. In Negima! Magister Negi Magi, Sakurazaki Setsuna is a bodyguard of sorts who is constantly teased for her overprotective attitude and attraction to her charge, Konoka.
In Mayo Chiki!, when Kinjiro refuses to be touched by a girl, because he has gynophobia with embarrassing physical symptoms, his male friend loudly questions him if he is gay, much to Kinjiro's embarrassment.rather extreme admiration of Otonashi after his episode also gets played for laughs a lot. In Angel Beats! there is a running joke about Hinata saying something questionable and Otonashi asking him if he's gay.